Specialist – Klein Lab Department of Orofacial Sciences University of California, San Francisco
We are looking for an enthusiastic, innovative, skilled individual to join our lab focused on stem cell, craniofacial development, and gastrointestinal regeneration. The incumbent will conduct research in developmental biology and participate in the generation, expansion and maintenance of novel mouse lines using iGONAD and CRISPR/Cas9-based methods.
Job Responsibilities: Perform experiments and data analysis under the direction of PI supervisor and as part of a team. Tasks will include, but not be limited to, the execution and troubleshooting of protocols for the generation of transgenic mouse lines. In addition, the applicant will be expected to attend / present during regular lab meetings and to perform additional duties in the lab as needed.
Required Qualifications: • A terminal degree in cell/molecular biology or related sciences or must have at least ten or more years of laboratory experience. • Previous experience in working with mice. • Ability to organize data and keep detailed records. • Proficiency with the following software programs: Microsoft Suite Office, Adobe Photoshop, Adobe Illustrator, SE-Central, SnapGene. • Excellent communication skills (both oral and written) in English. • Flexibility and willingness to work on multiple projects. • Direct experience with mouse transgenesis: CRISPR/Cas9 mutagenesis (design, validation of sgRNA, ssDNA, ssODN), iGONAD, offspring characterization by PCR screening and sequencing, biological validation. • Direct experience working on E. coli recombinant protein expression and purification, and on protein biophysical characterization.
Preferred Qualifications: • PhD degree preferred. • Post-graduate training in field. • Prior experience (at least 8 years of experience) in mouse handling and genotyping. • Prior experience in general and advanced molecular biology techniques. • Prior experience in Fluorescence Activated Cell Sorting (flow cytometry and single cell sorting). • Prior experience in intestinal stem cell biology, notably generating and maintaining small and large mouse intestinal enteroids. • Prior experience with hESCs mutagenesis and intestinal organoid differentiation. • Prior experience in RNA sequencing techniques and data analysis. • Knowledge of UCSF policies and procedures. • Excellent organization skill and attention to detail. • Excellent time and task management skills. • Ability to work effectively in a team environment, and to work independently. • Strong analytical abilities and problem-solving skills. • Strong knowledge of dental mineralized tissue biology. • Ability to maintain comprehensive laboratory notebook of experiments done on a daily basis. • Demonstrated ability to quickly learn and teach new techniques.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary and rank will be commensurate with the applicants experience and training.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.