The Bondy-Denomy lab is seeking an Specialist to join our group and contribute to new and ongoing projects. Our lab focuses on CRISPR-Cas immune systems and their natural functions in bacteria. We are also interested in discovering new phage-host interactions, developing new tools stemming from both Type I and II CRISPR-Cas systems and new applications for these systems in microbes (see Bondy-Denomy et al., Nature 2015, Rauch et al., Cell 2017, Marino et al. Science 2018, Jiang et al. Mol Cell 2018). The candidate will be working on a new large-scale project to systematically investigate the functions of phage-encoded small proteins in bacteria. The candidate will receive intensive training in developing high-throughput sequencing applications for conducting massively-parallelized biological assays. Depending on their progress, the candidate will also gain extensive experience in high-throughput protein purification and immunoprecipitation techniques. The candidate will be the primary lab scientist on the project and will additionally perform all routine microbiology and molecular biology techniques, including bacterial and bacteriophage manipulation, cloning, strain verification, cataloguing and management etc. An exceptional candidate will lead their own high-throughput assay development, develop new ideas, be familiar with the literature, and participate in manuscript writing and publication, where appropriate. Additionally, the specialist will be expected to assist with managing day-to-day lab operations, and perform general lab duties and be a positive lab citizen.
Required Qualifications: • Degree in molecular genetics, microbiology, or related discipline • Excellent organizational and interpersonal communication skills (verbal and written) • Willingness and ability to learn new methods and skills for changing research priorities. • Ability to work independently and as a member of a research team. • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines. • Must have (or be in process of obtaining) a bachelor’s degree (or equivalent degree) or four years of research experience to be appointed at the Junior rank. • Must have (or be in process of obtaining) a master’s degree (or equivalent degree) or at least five to ten years of research experience to be appointed at the Assistant or Associate ranks. • Must have (or be in process of obtaining) a terminal degree (or equivalent degree) or at least ten or more years of research experience to be appointed at the Full rank.
Preferred Qualifications: • Prior experience with high-throughput sequencing • Prior experience in the design and assembly of DNA constructs, plasmids etc. • Prior experience in bacterial genome manipulation (i.e. gene knockouts, locus removal, introduction of chromosomal tags and point mutations) • Two or more years of recent, directly related laboratory work experience utilizing techniques or methods required by the position. • Experience with molecular and microbiology techniques (including DNA and RNA isolation, PCR, gel electrophoresis, cloning, bacterial transformation etc.). • Experience in microbiology techniques (culturing, aseptic technique, plasmid manipulation, DNA/RNA extractions, protein purification)
We are seeking at least a 1-year commitment. Depending on performance and workload, opportunities for independent research, mentorship of other trainees, and scientific writing will be available.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.