Research - Laboratory/Non-Laboratory, Staff/Administrative
Under the general supervision of a faculty investigator or laboratory supervisor, the Research Specialist II will focus on performing both routine assays and experimental techniques in the study HIV infection and the immune response. He/she will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
The candidate will be responsible for independently managing multiple ongoing research projects simultaneously, analyzing data, generating figures to summarize the data, and presenting the data to a faculty investigator in a clear and concise way and in a timely manner. Given clear directives he/she is expected to have the ability to forward research endeavors quickly and efficiently will little supervision.
Specific Duties & Responsibilities
Perform various biological assays to explore mechanisms influencing viral outcome including but not limited to: Nucleic acid extractions and purifications, gel electrophoresis, real-time PCR, next-generation library preparation, tissue culture, flow cytometry, western blotting, etc.
Utilize various software to facilitate analyses including but not limited to: sequencing analysis, image analysis (Image J), and statistical analyses. R and Python knowledge are a plus.
Rigorous documentation of experimental planning, experimental methods, and experimental results updated on a daily basis. Responsible for operation of lab computers for data entry. Maintain accurate data records and laboratory notebook.
Monitoring and maintaining an inventory of blood products, cell lines, and nucleic acid samples. Requires excellent attention to detail and organizational skills. Maintains accurate data records.
Along with the faculty investigator or senior lab staff, works to develop and institute protocols for routine laboratory analyses. Maintains and improves training procedures and is responsible for training entry-level employees on relevant techniques.
Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, spectrophotometer, light microscope, plate washer and/or utilize biological safety cabinet. This person may also utilize sophisticated instrumentation such as flow cytometer, luminometer, thermal cycler, Lightcycler, etc.
Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures.
Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Additional responsibilities related to the daily laboratory operations will be assigned as needed.
Bachelor's Degree, in biology, chemistry, or other related discipline.
Requires a minimum of 2 years' laboratory experience performing relevant techniques in a comparable setting required.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula:
* JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job. *
Master's Degree in Biology
Special Knowledge, Skills, and Abilities
Basic to Advanced competency in R, including experience in, but not limited to, statistical analysis, gene expression analysis, and graphical data representation. Bash scripting experience to utilize current open-source bioinformatics pipelines. Basic Microsoft Office experience. Basic techniques in virology, immunology, and molecular biology.
Physical Requirements: Sitting, standing and walking for extended period. Reaching by extending hand(s) or arm(s) in any direction. Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s). Ability to move standard equipment through a hospital or clinical/laboratory environment.
Classified Title: Research Specialist II Working Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $38,920 - $53,520; Commensurate with Experience Employee group: Full Time Schedule: Monday-Friday, 9:30am- 6pm, Up to 37.5 hrs/ week Exempt Status: Exempt Location: 04-MD:School of Medicine Campus Department name: 60000203-SOM DOM ID Hepatitis Center Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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