The Stanford University School of Medicine Department of Biochemistry seeks a full-time Life Science Research Professional 1 (LSRP) into a (2 Year Fixed Term) to work in a lab specializing in the detection and functional probing of RNA structures. You will be work with a Stanford MD/PhD student to investigate the mechanisms of sub-cellular localization of mRNA in oligodendrocytes. Your work will focus on generating and maintaining primary oligodendrocyte cell cultures that you will use for a variety of imaging and sequencing-based RNA experiments. Your work will contribute to at least two on-going high-impact projects in the lab.
If you have strong experience in mammalian cell culture, , and a desire to work in a collaborative, well-resourced lab environment, then please join us in pushing the field of RNA localization forward!
Department of Biochemistry: Stanford Biochemistry is a diverse and inclusive community dedicated to fundamental discovery in biological science through basic research and training. We are united by the common view that transformative advances in biology come through a molecular understanding of biological systems. Our core goals are to perform foundational research, to mentor and train scientists and scientific leaders, and to foster a strong scientific community. Our research ranges from molecular structure to organismal biology as we strive to uncover new biological principles and to impact human disease. Central to our research mission is our long-standing culture of sharing and strong sense of community and collegiality. We mix members of our laboratories together throughout the Department in order to foster cross pollination of ideas and approaches. We promote a supportive environment that values different people and perspectives.
Your responsibilities will include:
Using your experience in mammalian cell culture to regularly isolate and culture primary rodent oligodendrocytes.
Single-molecule RNA imaging in fixed and living cell samples through spinning-disc confocal microscopy.
Statistical analysis of fluorescence microscopy data.
RNA extraction, RT-qPCR, and library preparation for high-throughput sequencing.
Accurate and meticulous laboratory notebook record keeping.
Independently conduct experiments; maintain detailed records of experiments and outcomes.
Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
Contribute to publication of findings. Participate in the preparation of written documents, including procedures, presentations, and proposals.
Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
*- Other duties may also be assigned.
How to apply: If you are interested in joining us, please click the Apply for Job button to submit a cover letter and resume along with an online application.
Bachelor's in Molecular Biology, Cell Biology, Computational Biology, Biology, or similar field.
At least two years of experience in mammalian cell culture, preferably in the context of stem cells.
Being comfortable working with small animals.
Experience in immunofluorescence staining, confocal microscopy, and RNA molecular biology (e.g. RNA extraction, RT-qPCR).
Experience with high-throughput sequencing library preparation is desired but not required.
Experience in basic computational approaches (i.e. programming or scripting experience) is desired but not required.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
Ability to maintain detailed records of experiments and outcomes.
General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
Ability to work under deadlines with general guidance.
Excellent organizational skills and demonstrated ability to accurately complete detailed work.
Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
Must have correctible vision to perform duties of the job.
Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
Ability to lift heavy objects weighing up to 50 pounds.
Ability to work in a dusty, dirty, and odorous environment.
Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
May require working in close proximity to blood borne pathogens.
May require work in an environment where animals are used for teaching and research.
Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
May require extended or unusual work hours based on research requirements and business needs.
Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.
Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.
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Located between San Francisco and San Jose in the heart of Silicon Valley, Stanford University is recognized as one of the world's leading research and teaching institutions. Leland and Jane Stanford founded the University to "promote the public welfare by exercising an influence on behalf of humanity and civilization." Stanford opened its doors in 1891, and more than a century later, it remains dedicated to finding solutions to the great challenges of the day and to preparing students for leadership in a complex world. The University's thriving diverse community is comprised of nearly 7000 undergraduate students, 12000 graduate students, 2000 faculty members, 1900 postdoctoral scholars, and over 11,000 academic and administrative staff in seven schools including several interdisciplinary research centers and institutes. The campus spreads over 8000 contiguous acres and nearly all undergraduates live on campus. Stanford offers bachelor's and master's degrees in addition to doctoral degrees (PhD, MD, DMA and JD) plus a number of professional and continuing education programs and certifications. More at http://facts.stanford.edu and http://www.stanford.edu. Stanford is an equal empl...oyment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Stanford also welcomes applications from others who would bring additional dimensions to the University’s research, teaching and clinical missions.